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目的研究Fas相关死亡结构域(FADD)蛋白在膀胱尿路上皮癌(BUC)组织的表达,并探讨其与肿瘤生物学行为之间的关系。方法采用免疫组化SP法检测68例BUC及13例正常膀胱黏膜组织FADD蛋白表达。原位杂交法检测其中34例BUC组织FADD mRNA表达水平。结果正常膀胱黏膜FADD蛋白呈阳性表达。FADD的阳性率在不同组织学分级、不同临床分期的BUC组织中差异均有极显著性(χ2=15.38、11.34,P<0.01);复发组与未复发组FADD的阳性率分别为36.84%和53.33%,差异无显著意义(2χ=1.85,P>0.05)。BUC组织中FADD mRNA阳性率为44.12%,FADD蛋白阳性率为47.06%,两者差异无统计学意义(χ2=0.06,P>0.05)。结论FADD表达异常在BUC的发展中可能起着重要作用,FADD阳性表达与BUC病理分级、浸润关系密切,但与BUC复发无关。
Objective To study the expression of Fas-associated death domain (FADD) protein in bladder urothelial carcinoma (BUC) tissues and to explore its relationship with tumor biological behavior. Methods Immunohistochemical SP method was used to detect the protein expression of FADD in 68 cases of BUC and 13 cases of normal bladder mucosa. In situ hybridization was used to detect the expression of FADD mRNA in 34 cases of BUC tissues. Results The positive expression of FADD protein in normal bladder mucosa was observed. The positive rates of FADD were significantly different in different histological grades and different clinical stages of BUC (χ2 = 15.38, 11.34, P <0.01). The positive rates of FADD in relapsed and non-relapsed patients were 36.84% and 53.33%, the difference was not significant (2χ = 1.85, P> 0.05). The positive rate of FADD mRNA in BUC was 44.12% and the positive rate of FADD was 47.06%. There was no significant difference between the two groups (χ2 = 0.06, P> 0.05). Conclusion The abnormal expression of FADD may play an important role in the development of BUC. The positive expression of FADD is closely related to the pathological grade and infiltration of BUC, but not to the recurrence of BUC.