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研究了远缘种鲤的微卫星引物对黄鳝的适用性。结果显示,31对鲤微卫星引物中有11对引物能对黄鳝DNA模板扩增出特异性带谱。每对引物扩增的等位基因数在3~13个,平均每个位点有5.6个等位基因,显示了较高的多态性。其中引物P1最理想,其PCR扩增产物能区分来自湖南、广东和孟加拉3个不同地域的黄鳝种群。应用微卫星技术对3个不同地域的黄鳝基因组DNA多态性分析结果显示,湖南、广东和孟加拉黄鳝群体内平均相似率依次为95.5%,95.8%和93.5%,平均变异度依次为0.045,0.042,0.063。群体间的相似率及变异度分析显示:湖南黄鳝和广东黄鳝群体间平均相似率为91.0%,变异度为0.045;湖南黄鳝和孟加拉黄鳝群体间平均相似率和变异度分别为55.7%和0.443;广东黄鳝和孟加拉黄鳝群体间平均相似率和变异度分别为58.6%和0.414。综合微卫星分析结果、黄鳝的外形特征及地理位置,可以推测,广东黄鳝与湖南黄鳝为同一个生物种的不同地理种群,而孟加拉黄鳝为同属中另一个种。
The applicability of microsatellite primers from distant species carp to rice field eel was studied. The results showed that 11 pairs of microsatellite primers of 31 pairs of common carp could amplify the specific bands of rice eel DNA template. The number of alleles amplified by each primer ranged from 3 to 13 with an average of 5.6 alleles per locus, indicating a high polymorphism. Among them, the primer P1 was the best, and its PCR amplification products could distinguish the eel population from 3 different areas in Hunan, Guangdong and Bangladesh. Microsatellite DNA analysis of genomic DNA in rice field eel from three different regions showed that the average similarity rates in populations of Eel in Hunan, Guangdong and Bengal were 95.5%, 95.8% and 93.5%, respectively, and the average variation rates were 0.045 and 0.042 , 0.063. The analysis of similarity and variability showed that the average similarity between Hunan eel and Guangdong eel was 91.0% and the variation was 0.045. The average similarity and variability between Hunan eel and Bengal were 55.7% and 0.443, respectively. The average similarity and variability of Guangdong eel and Bengal eel were 58.6% and 0.414, respectively. Based on the results of microsatellite analysis and the shape and geographical location of the eel, it can be speculated that Guangdong eel and Hunan eel are different geographical populations of the same species, while Bengal eel is another species of the same genus.