八十年前,Ehrlich曾设想用肿瘤抗体携带白喉毒素治疗肿瘤。长期来使用肿瘤抗原免疫动物,取其免疫血清治疗肿瘤,疗效甚微。主要问题是肿瘤抗原提纯困难,获得的抗体不纯。直至1975年Kohler和Milstein创用羊红细胞免疫小鼠,免疫后取出小鼠脾,制成能大量产生抗体的B细胞悬液,与小鼠多发性骨髓瘤细胞株细胞融合产生的杂交瘤,具有其亲代的特点,既能产生抗羊红细胞抗体,又能不断增 Eighty years ago, Ehrlich had envisioned the use of tumor antibodies to carry diphtheria toxin to treat tumors. Long-term use of tumor antigen immunized animals, take its immune serum treatment of tumors, with little effect. The main problem is the difficulty of purification of tumor antigens, obtained antibodies impure. Until 1975, Kohler and Milstein created a sheep red blood cell immunized mice, immunized mice spleen removed to make a large number of antibody-producing B cell suspension, and mouse multiple myeloma cell line fusion of the resulting hybridoma, with The characteristics of their parents, both to produce anti-sheep red blood cell antibodies, but also continue to increase