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[目的]探讨微波辐照对小胶质细胞活化的影响及JAKs(janus activated kinase)家族蛋白质差别激活。[方法]90mW/cm2微波辐照离体培养的N9小胶质细胞,在辐照前、后不同时间采用细胞流式计数的方法观察N9细胞CD11b的表达情况,采用western blot检测JAKs家族蛋白质磷酸化水平的变化。[结果]微波辐照后3h阳性表达CD11b的小胶质细胞明显增加,6h达到高峰,并一直持续到辐照后24h(P均<0.05)。JAK1蛋白磷酸化水平于辐照后3h开始明显升高(P<0.05),一直持续到24h;JAK2在辐照后1～6h逐渐升高,并在6h达最高峰(P<0.01);JAK3在辐照后6h明显升高(P<0.01),到24h后仍然高于对照水平(P<0.05)。[结论]微波辐照可明显诱导小胶质细胞活化,使小胶质细胞JAK1、JAK2、JAK3出现差别激活。JAKs家族蛋白可能参与了微波辐照诱导的小胶质细胞活化。
[Objective] To investigate the effect of microwave irradiation on the activation of microglia and the differential activation of JAKs family proteins. [Method] The N9 microglial cells cultured in vitro were irradiated with 90mW / cm2 microwave. The expression of CD11b in N9 cells was observed by flow cytometry at different time before and after irradiation. Western blot was used to detect the phosphorylation of JAKs family proteins The level of change. [Result] The number of microglial cells expressing CD11b at 3h after microwave irradiation increased significantly, reached the peak at 6h, and continued to 24h after irradiation (all P <0.05). The level of JAK1 phosphorylation began to increase at 3h after irradiation (P <0.05), and continued until 24h; JAK2 increased gradually from 1h to 6h after irradiation and peaked at 6h (P <0.01); JAK3 6h after irradiation (P <0.01), still higher than the control level after 24h (P <0.05). [Conclusion] The microglia could be induced obviously by microwave irradiation, and the differential activation of JAK1, JAK2 and JAK3 in microglia could be observed. JAKs family of proteins may be involved in microglial activation induced by microwave irradiation.