论文部分内容阅读
观察红杉醇(sequoyitol,Seq)对2型糖尿病大鼠主动脉内皮型一氧化氮合酶(eNOS)及NADPH氧化酶4(NOX4)表达的影响。采用小剂量链脲佐菌素(STZ,35 mg·kg-1)加高脂高糖饮食诱导2型糖尿病动物模型,灌服Seq(12.5、25及50 mg·kg-1·d-1)治疗6周后,于末次给药后测定空腹血糖。使用离体主动脉环灌流法,观察主动脉环对乙酰胆碱和硝普钠的舒张反应,HE染色观察主动脉病理学变化,放射免疫法检测血清胰岛素水平;比色法测定主动脉总抗氧化能力(T-AOC)、丙二醛(MDA)及一氧化氮(NO)含量;real-time PCR、Western blotting及免疫组化检测主动脉eNOS及NOX4 mRNA和蛋白表达。结果显示,红杉醇给药6周后能明显降低糖尿病大鼠空腹血糖,减轻胰岛素抵抗,改善主动脉内皮依赖性舒张功能,上调主动脉eNOS的表达,提高T-AOC及NO水平,降低NOX4的表达及MDA含量。上述结果表明,Seq可能通过下调NOX4表达、上调eNOS表达进而对糖尿病大鼠主动脉内皮功能损伤产生保护作用。
To observe the effect of sequoyitol (Seq) on the expression of endothelial nitric oxide synthase (eNOS) and NADPH oxidase 4 (NOX4) in type 2 diabetic rats. The animal model of type 2 diabetes was induced by using low-dose streptozotocin (STZ, 35 mg · kg-1) and high-fat and high-sucrose diet. Seq (12.5,25 and 50 mg · kg-1 · d-1) After 6 weeks of treatment, fasting plasma glucose was measured after the last dose. Aortic ring perfusion method was used to observe the relaxation of aortic rings against acetylcholine and sodium nitroprusside, HE staining was used to observe the pathological changes of the aorta, and the levels of serum insulin were measured by radioimmunoassay. The total anti-oxidative capacity (T-AOC), malondialdehyde (MDA) and nitric oxide (NO) were detected by real-time PCR. Western blotting and immunohistochemistry were used to detect the mRNA and protein expression of eNOS and NOX4 in the aorta. The results showed that after 6 weeks of administration, the fasting blood glucose, the insulin resistance, the aortic endothelium-dependent vasodilatation, the aortic eNOS expression, the T-AOC and NO levels, and the NOX4 Expression and MDA content. The above results show that Seq may protect the aortic endothelial dysfunction in diabetic rats by down-regulating the expression of NOX4 and up-regulating the expression of eNOS.