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详细研究了半胱氨酸与钴（Ⅱ）在ＮＨ４ＣＬＮＨ４ＯＨ介质中的相互作用，建立了快速测定半胱氨酸的分光光度法，方法检出限２８×１０－６ｍｏｌ·Ｌ－１，线性范围０～２０×１０－４ｍｏｌ·Ｌ－１，摩尔吸光系数７９８×１０３Ｌ·ｍｏｌ－１·ｃｍ－１。用于尿液中半胱氨酸的测定，结果满意。
The interaction between cysteine and cobalt (Ⅱ) in NH4CL-NH4OH medium was studied in detail. A spectrophotometric method was developed for the rapid determination of cysteine. The detection limit was 2. 8 × 10-6mol·L-1 , Linear range of 0 ~ 2 0 × 10-4mol·L-1, molar extinction coefficient of 798 × 103L · mol-1 · cm-1. For the determination of cysteine in urine, the results are satisfactory.