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以１次、半巢式及多重ＰＣＲ方法扩增５８例淋巴细胞白血病ＩｇＨ及ＴＣＲＶγＩ－Ｊγ克隆性基因重排。结果显示：多重ＰＣＲ和１次ＰＣＲ的敏感度为１０￣（－４）～１０￣（－５）水平；半巢式ＰＣＲ敏感度可达１０￣（－５）～１０￣（－６）水平。ＩｇＨ和ＴＣＲＶγＩ－Ｊγ重排阳性率为６７．２％和６２．０％；半巢式ＰＣＲ检测ＩｇＨ重排阳性率为７０．７％。上述结果表明：多重ＰＣＲ可同时检测两对目的基因，适于初治病例检测，而对缓解病例的检测则需采用敏感度较高的半巢式ＰＣＲ方法。
Fifty-eight cases of lymphocytic leukemia IgH and TCRVγI-Jγ clonal gene rearrangements were amplified by 1-step, semi-nested and multiplex PCR. The results showed that the sensitivity of multiplex PCR and primary PCR was 10 ~ (-4) ~ 10 ~ (-5), and the sensitivity of semi - nested PCR was about 10 ~ (-5) ~ 10 ~ (-6) . The positive rates of IgH and TCRVγI-Jγ rearrangements were 67.2% and 62.0%, respectively. The positive rate of IgH rearrangement detected by semi-nested PCR was 70.7%. The above results show that multiplex PCR can detect two pairs of target genes at the same time and is suitable for the detection of naive cases, while the detection of remission cases requires the use of highly sensitive semi-nested PCR.