目的 :研究热休克恢复期小鼠肝细胞热休克蛋白 (HSP70 )的表达。方法 :小鼠分别以 4 0、4 2、4 4、4 6℃热休克处理 30 min,恢复 8h;4 6℃热休克处理 30 min,然后分别于热休克后 2～ 72 h取肝 ,以免疫组化方法观察 HSP70的表达 ,并用体视学方法对阳性肝细胞体密度进行定量分析。结果 :4 2、4 4、4 6℃均能诱导肝细胞表达 HSP70 ,以 4 6℃组小鼠阳性肝细胞密度最大 ;4 6℃热休克后 8～ 12 h为 HSP70的合成高峰 (P<0 .0 1) ,72 h后基本降解消失。结论 :HSP70的迅速表达与缓慢降解对维持肝细胞的正常生理功能有重要作用。 Objective: To study the expression of heat shock protein (HSP70) in mouse liver cells during heat shock recovery. Methods: The mice were heat-shocked at 4 0,4 ​​2,4 4,4 6 ℃ for 30 min, resumed for 8 h, then treated with heat shock at 4 6 ℃ for 30 min, and then were harvested at 2 to 72 h after heat shock Immunohistochemistry was used to observe the expression of HSP70. The body density of hepatocytes was quantitatively analyzed by stereological methods. Results: 4, 4, 4, 4 and 6 6 ℃ all induced HSP70 expression in hepatocytes, and the highest density of positive hepatocytes was observed in 4 6 ℃ group. The synthesis peak of HSP70 was observed in 8 ~ 12 h after heat shock at 46 ℃ (P < 0 .0 1), 72 hours after the basic degradation disappeared. Conclusion: The rapid expression and slow degradation of HSP70 play an important role in maintaining the normal physiological function of hepatocytes.