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目的观察维拉帕米对冻僵大鼠能量代谢和氧化代谢的影响。方法40只Wistar雄性大鼠随机分成5组。对照组在室温20℃下饲养。冻僵组为-30℃冷暴露80 min。干预1、2、3组分别依次预先30 min腹腔注射维拉帕米5、10、20mg/kg,然后于-30℃冷暴露80 min,室温复温4 h,麻醉后腹主动脉采血,测定血糖、乳酸(LD)、丙二醛(MDA)、谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)活力。摘取肝脏,测定肝脏中的MDA含量、GSH和SOD活力。结果冻僵组大鼠血糖低于对照组(P<0.05),冻僵组血清中LD、MDA、GSH和肝脏中LD,MDA、GSH含量及SOD活力均高于对照组(P<0.05)。3个干预组大鼠肛温温差、血糖、血清与肝脏中MDA含量和GSH活力均低于冻僵组(P<0.05)。干预2、3组血清中LD含量和肝脏中SOD活力均低于冻僵组(P<0.05)。结论维拉帕米不仅能够减轻能量代谢中无氧呼吸废物对机体的损伤,同时还能够降低冷暴露时对机体的氧化损伤。
Objective To observe the effects of verapamil on energy metabolism and oxidative metabolism in frozen rats. Methods Forty male Wistar rats were randomly divided into five groups. The control group was housed at room temperature of 20 ° C. Frozen group was -30 ℃ cold exposure 80 min. Intervention groups 1, 2, and 3 were given intraperitoneal injection of verapamil 5,10 and 20 mg / kg 30 min before injection, then exposed to -30 ℃ for 80 min, rewarmed for 4 h at room temperature, Blood glucose, lactate (LD), malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) activity. Liver was harvested, MDA content in liver, GSH and SOD activity were measured. Results Compared with the control group, the blood glucose in the frozen group was significantly lower than that in the control group (P <0.05). The contents of LD, MDA, GSH in serum and LD, MDA, GSH and SOD in the frozen group were higher than those in the control group (P <0.05). The analgesic temperature difference, blood glucose, MDA content and GSH activity in serum and liver of the three intervention groups were all lower than those in the frozen group (P <0.05). Intervention group 2, 3 serum LD and liver SOD activity were lower than the frozen group (P <0.05). Conclusion Verapamil can not only reduce the body’s damage caused by oxygen-free respiratory waste in energy metabolism, but also reduce the oxidative damage to the body during cold exposure.