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采用孔蛋白(MspA)和双肉豆蔻磷脂酰胆碱(DMPC)在玻碳(GC)基底表面成功构建有仿生特性的纳米通道膜,同时将葡萄糖氧化酶(GOD)修饰于膜上.使用循环伏安法研究GOD/MspA-DMPC/GC电极的GOD直接电化学过程以及其对氧气和葡萄糖的响应.研究发现,MspA与DMPC形成的仿生纳米通道膜内,GOD在接近生物体系FAD/FADH2标准电位处实现了自身两质子、两电子表面控制的电化学反应.MspA与DMPC的仿生纳米通道膜体系为GOD提供了理想活性环境.
Biomimetic nanochannels were successfully constructed on the surface of glassy carbon (GC) substrates by using MspA and DMPC, and glucose oxidase (GOD) was modified on the membrane. Voltammetric study of the GOD direct electrochemical process and its response to oxygen and glucose at GOD / MspA-DMPC / GC electrode revealed that the GOD was close to the FAD / FADH2 standard of biological system in the biomimetic nanochannel membrane formed by MspA and DMPC Potential at the realization of its own two protons, two electronic surface-controlled electrochemical reaction.MspA and DMPC bionic nanochannel membrane system provides an ideal environment for GOD.