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以4个不结球白菜新组合为试材,通过室内人工接种和分子标记辅助选择的方法,对其根肿病抗性进行了鉴定,以期为不结球白菜抗根肿病分子标记筛选和新品种选育提供依据。结果表明:不结球白菜组合‘5-3’对根肿菌4号生理小种表现为高抗,‘5-2’表现为耐病,‘5-1’和‘5-4’表现为感病;利用5对根肿病抗性相关的引物对材料进行检测,结果显示组合‘5-1’在3对引物标记下均扩增出条带,‘5-3’和‘5-4’在2对引物下扩增出条带,‘5-2’在1对引物标记下扩增出条带。人工接种鉴定结果与分子标记鉴定结果存在差异,高抗材料‘5-3’在引物TCR05和TCR09下均有单一条带,而接种鉴定为感病的‘5-1’也同样在这2对引物下扩增出阳性条带,说明应用于不结球白菜根肿病抗性检测的分子标记的有效性需进一步提高,实践中应采用多种抗病鉴定相结合的方法。
Four new combinations of non-heading Chinese cabbage were used as materials to identify their clubroot disease resistance by artificial inoculation and molecular marker-assisted selection in order to screen for molecular markers of resistance to clubroot in non-heading Chinese cabbage Breeding new species provide the basis. The results showed that ’5-3’ of non-heading Chinese cabbage showed high resistance to R. solani 4 race, ’5-2’ showed resistance, ’5-1’ and ’5-4’ Five pairs of clubroot disease resistance-related primers were used to test the materials. The results showed that the combination of ’5-1’ bands was amplified by 3 pairs of primers and the bands of ’5-3’ and ’5-4’ Two pairs of primers amplified bands, ’5-2’ in a pair of primer-labeled bands were amplified. There was a difference between the results of artificial inoculation and the identification of molecular markers. High resistance material ’5-3’ had a single band under the primers TCR05 and TCR09, while the ’5-1’ The positive bands were amplified by primer, which indicated that the molecular marker applied to the detection of clubroot disease resistance in non-heading Chinese cabbages needs to be further improved. In practice, a combination of disease resistance identification methods should be used.