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2009-2010年春季,先后从甘肃、四川、陕西和西藏四省的29个县(市)采集到1 391份小麦条锈病标样,繁殖获得961个菌株,利用SSR分子标记进行群体遗传分析结果表明:甘肃天水、平凉和陇南,陕西宝鸡及汉中,四川阿坝和广元等地条锈菌的遗传多样性比较丰富,而四川宜宾及凉山、西藏林芝的遗传多样性水平相对较低。利用Arlequin软件中的AMOVA方法分析结果表明,小麦条锈菌的遗传变异主要存在于群体内部。内地各种群之间菌源交流频繁(Nm>4),西藏与内地菌源交流很少(Nm<1)。采用Structure及聚类分析表明,陕西宝鸡与甘肃平凉间,陕西汉中、甘肃陇南、甘肃天水及四川广元间,存在着频繁的菌源交流关系,四川宜宾和凉山与四川阿坝、陕西汉中和甘肃陇南间存在着菌源交流关系。而西藏与内地间几乎没有菌源交流。初步认为西藏林芝小麦条锈菌可能是一个相对独立的遗传群体。
In the spring of 2009-2010, 1 391 wheat stripe rust samples were collected from 29 counties (cities) of Gansu, Sichuan, Shaanxi and Tibet provinces and 961 strains were obtained. SSR markers were used for population genetic analysis The results showed that the genetic diversity of Puccinia striiformis in Tianshui, Pingliang and Gansu, Baoji and Hanzhong in Shaanxi, Aba and Guangyuan in Sichuan was abundant, while the genetic diversity in Yibin and Liangshan in Sichuan and Nyingchi in Tibet was relatively low. The results of AMOVA analysis using Arlequin software showed that the genetic variation of wheat stripe rust mainly existed in the population. The bacteria in the Mainland have frequent exchanges of bacteria (Nm> 4), and there is very little communication between the bacteria in Tibet and the Mainland (Nm <1). Structure and cluster analysis showed that there were frequent exchanges between bacteria sources in Baoji of Shaanxi Province, Pingliang of Gansu Province, Hanzhong of Shaanxi Province, Longnan of Gansu, Tianshui of Gansu and Guangyuan of Sichuan Province. Sichuan, Yibin and Liangshan and Sichuan Aba, Hanzhong of Shaanxi and Longnan of Gansu Between the existence of bacteria exchange relations. There is almost no bacterial source exchange between Tibet and the Mainland. It is preliminarily thought that the strain of wheat stripe rust in Linzhi of Tibet may be a relatively independent genetic group.