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目的:探讨急性髓细胞白血病(AML)免疫分型和FAB分型的关系。方法:对93例初治AML进行细胞形态学检查和免疫分型检查,免疫分型采用流式细胞术(FCM),并对2种检查结果进行分析。结果:93例AML中M1亚型1例,M2亚型68例,M3亚型13例,M4亚型3例,M5亚型4例,未分类4例;单表型74例,伴淋系抗原表达12例,双表型5例,未表型2例;细胞形态学确诊AML72例,诊断准确率为77.4%;免疫分型确诊AML91例,FAB分型结合免疫分型使诊断的准确率达到97.8%。结论:免疫分型在鉴别AML各亚型和杂合性白血病(HAL)方面具有重要作用,细胞形态学结合免疫分型可提高诊断准确率,弥补FAB分型的不足之处。
Objective: To investigate the relationship between immunophenotyping and FAB typing in acute myeloid leukemia (AML). Methods: 93 cases of untreated AML were examined by cell morphology and immunophenotyping. Immunofluorescence was performed by flow cytometry (FCM) and the results of two kinds of tests were analyzed. Results: There were 1 case of M1 subtype in 93 cases, 68 cases of M2 subtype, 13 cases of M3 subtype, 3 cases of M4 subtype, 4 cases of M5 subtype, 4 cases not classified; 74 cases of single phenotype with lymph node Antigen expression in 12 cases, 5 cases of dual phenotype, 2 cases of non-phenotype; 72 cases diagnosed AML 72 cases diagnosed by cell morphology; AML diagnosis of 91 cases, FAB typing combined with immunophenotyping diagnostic accuracy Reached 97.8%. Conclusion: Immunophenotyping plays an important role in differentiating AML subtypes and heterozygous leukemia (HAL). Cytohistomorphology and immunophenotyping can improve diagnostic accuracy and make up for deficiencies in FAB typing.