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用超滤和纳滤膜分离技术提取发酵液中的阿卡波糖。发酵液通过超滤膜除去杂质,然后将超滤液通过纳滤膜除盐和浓缩。筛选了不同型号的超滤膜和纳滤膜,并分别优化操作条件,得到适宜的工艺条件:选用ULT-1超滤膜,阿卡波糖发酵液的进料浓度3 g/ml,操作压力0.26～0.28 MPa,操作温度25℃;选用STM-2纳滤膜,操作压力0.6～0.8 MPa,操作温度25℃,料液浓缩倍数为6倍。应用上述条件提取发酵液中的阿卡波糖,提取率为62.2%,较原工艺提高了约20%,浓缩液中阿卡波糖含量高于98.8%。
Extraction of Acarbose from Fermentation Broth by Ultrafiltration and Nanofiltration Separation Technology. Fermentation broth through the ultrafiltration membrane to remove impurities, and then the ultrafiltrate through the nanofiltration membrane desalination and concentration. Different types of ultrafiltration membranes and nanofiltration membranes were screened, and the operating conditions were optimized respectively to obtain the suitable technological conditions: ULT-1 ultrafiltration membrane was used, the feeding concentration of acarbose fermentation broth was 3 g / ml, the operating pressure 0.26 ~ 0.28 MPa, operating temperature 25 ℃; the choice of STM-2 nanofiltration membrane, the operating pressure of 0.6 ~ 0.8 MPa, operating temperature 25 ℃, feed concentration times 6 times. Acarbose was extracted from fermentation broth under the above conditions. The extraction rate was 62.2%, which was about 20% higher than the original one. The content of acarbose in the concentrate was higher than 98.8%.