论文部分内容阅读
目的探讨黄连碱对人乳腺癌细胞株MDA-MB-231细胞周期和增殖的作用及相关机制。方法 MTT法和瑞-姬氏染色法分别检测黄连碱对MDA-MB-231细胞增殖的影响,流式细胞术检测细胞周期分布,RT-PCR检测细胞周期相关基因包括细胞周期蛋白依赖性激酶4(CDK4)、CDK6、p21及p27mRNA的表达变化。结果黄连碱可明显抑制MDA-MB-231细胞的增殖;黄连碱处理细胞后G0/G1期细胞比例减少,而S期和G2/M期细胞比例增高(P<0.05或P<0.01);黄连碱可上调p21mRNA的表达、下调CDK4mRNA和CDK6mRNA的表达(P<0.01),而对p27mRNA水平无显著影响。结论黄连碱在体外能通过诱导S期及G2/M期周期阻滞来抑制MDA-MB-231细胞的增殖;其机制可能与p21mRNA表达上调导致CDK4mRNA及CDK6mRNA减少有关。
Objective To investigate the effects and mechanisms of berberine on the cell cycle and proliferation of human breast cancer cell line MDA-MB-231. Methods The effects of Coptis alkaloid on the proliferation of MDA-MB-231 cells were detected by MTT assay and Reiki-Giemsa staining. The cell cycle distributions were detected by flow cytometry. The expressions of cyclin-dependent kinase 4 (CDK4), CDK6, p21 and p27 mRNA expression changes. Results Coptis alkaloid significantly inhibited the proliferation of MDA-MB-231 cells; the proportion of cells in G0 / G1 phase decreased after treated with Coptis chinensis, but increased in S phase and G2 / M phase (P <0.05 or P <0.01) Alkali can up-regulate the expression of p21mRNA and down-regulate the expression of CDK4mRNA and CDK6mRNA (P <0.01), but not p27mRNA. Conclusions Coptisine can inhibit the proliferation of MDA-MB-231 cells by inducing S phase and G2 / M phase arrest in vitro. Its mechanism may be related to the decrease of CDK4 mRNA and CDK6 mRNA induced by up-regulation of p21 mRNA.