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用 8 0个 10bp随机引物对我国新疆核桃、华北山地核桃、秦巴山地核桃和西藏高地核桃 4个不同地理生态型的 2 0个样本进行RAPD扩增反应 .选用了清晰度高、重复性好的 2 4个引物用于进一步的分析 ,共扩增出 2 2 7个RAPD片段 ,具多态性的片段有 16 8个 ,占总扩增片段的 74% ,平均每个引物扩增片段为 9 46个 ,扩增片段长度为2 2 0～ 40 72bp .其中 ,西藏核桃类群的特有RAPD片段占有很大比例 .依据扩增结果 ,计算遗传距离 ,采用UPGMA方法构建聚类树状图 .结果表明 :新疆核桃为我国栽培核桃的一个地理生态型 ;华北山地核桃和秦巴山地核桃应属一个地理生态型下的两个不同亚群 ;西藏高地核桃不仅在聚类树状图上被划为另一大类 ,而且遗传距离与其它地理生态型也相差很远 .西藏高地核桃是作为一个地理生态型 ,还是作为一个独立的植物种有待进一步深入研究
A total of 80 random primers were used to amplify RAPD in 20 geographically ecotypes of walnut from China, walnut from North China, walnut from North China, walnut from Qinba Mountain and walnut from Tibet Highland, and high resolution and good reproducibility Of 24 primers were used for further analysis. A total of 227 RAPD fragments were amplified with 16 8 polymorphic fragments, accounting for 74% of the total amplified fragments. The average amplification range of each primer was 9 46, the amplified fragment length of 220 ~ 40 72bp .In which, Tibet walnut group unique RAPD fragments occupy a large proportion of the amplification results based on the calculation of genetic distance, UPGMA method to construct a cluster dendrogram .Results The results showed that: Xinjiang walnut is a geographical ecotype of cultivated walnut in China; walnut in North China and walnut in Qinba Mountain belong to two different subgroups in a geographical ecotype; Tibet highland walnut is not only classified as Another kind, and the genetic distance is far away from other types of geography.Hubei Heights walnut as a geographical or ecological, or as an independent species of plants to be further in-depth study