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目的:建立一种检测人血浆中奥拉西坦的液相色谱-质谱联用(LC-ESI-MS)的方法。方法:加入吡拉西坦为内标,血浆中的药物用甲醇沉淀法去蛋白。采用Agilent Zorbax SB-C18(2.1 mm×100 mm,3.5μm)柱,流动相为甲醇-0.1%甲酸溶液(5:95,v/v)。采用电喷雾离子源(ESI),正离子检测,选择性离子监测(selected ion mon itoring,SIM)方式进行定量分析奥拉西坦,监测离子峰分别是m/z 158.5(奥拉西坦)和m/z 142.5(内标)。结果:血浆中奥拉西坦检测方法的线性范围为0.1~8μg/m l,最低定量限为0.1μg/m l,平均回收率在91.5%~96.4%之间,日内和日间精密度RSD都小于9%。结论:本方法简单、灵敏、快速,可用于药物动力学研究。
Objective: To establish a method for the determination of oxiracetam in human plasma by liquid chromatography-mass spectrometry (LC-ESI-MS). Methods: Piracetam was added as an internal standard, and the plasma drug was deproteinized by methanol precipitation. An Agilent Zorbax SB-C18 (2.1 mm × 100 mm, 3.5 μm) column was used with a mobile phase of methanol-0.1% formic acid (5:95, v / v). Quantitative analysis of oxiracetam was carried out by electrospray ionization (ESI), positive ion detection and selected ion monitoring (SIM). The monitored ion peaks were m / z 158.5 (oxiracetam) and m / z 142.5 (internal standard). Results: The linear range of oxiracetam in plasma was 0.1-8 μg / ml with the lowest limit of quantitation of 0.1 μg / ml and the average recovery was between 91.5% and 96.4%. The intra-day and inter-day RSDs were less than 9%. Conclusion: The method is simple, sensitive and rapid and can be used for pharmacokinetic studies.