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用光镜、组织化学、免疫组织化学、透射电镜(TEM)、扫描电镜(SEM)观察移植于新生鼠体内的子宫颈癌(U14)细胞、6例人类乳腺单纯癌、4例宫颈鳞癌细胞浸润及转移情况。结果表明:①在TEM下进一步证实了体内癌细胞浸润时能释放溶酶体,并吞噬坏变的组织碎片形成吞噬泡。②浸润的癌细胞四周有薄层坏死带(浸润性癌溶现象)。③部分硬癌、单纯癌的“癌巢”是脉管的痊栓,它可发展成癌巢。鉴别癌巢与癌栓对临床有重要意义。④针穿刺对癌性腹水是不安全的。
Light microscopy, histochemistry, immunohistochemistry, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to observe cervical cancer (U14) cells implanted in neonatal rats, 6 cases of human breast cancer, and 4 cases of cervical squamous cancer cells. Infiltration and transfer conditions. The results showed that: 1 It was further confirmed by TEM that lysosomes could be released when cancer cells were infiltrated in vivo, and phagocytic vacuoles were formed by phagocytosis. 2 Infiltrating cancer cells have a thin layer of necrotic zone (invasive cancerous phenomenon). 3 The “cancer nest” of hard cancers and simple cancers is a vascular thromboembolism that can develop into a cancerous nest. Identifying cancer nests and tumor thrombi is of great clinical significance. 4-needle puncture is not safe for cancerous ascites.