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目的研究新型雌激素受体(ER)β在肺腺癌和鳞癌组织中的表达,探讨其与肺癌临床病理学特征、吸烟和外周血雌激素水平的关系。方法采用免疫组织化学和免疫印迹技术检测ERβ在26例肺腺癌和38例肺鳞癌(共有37例含癌旁肺组织)及15例良性肺病变组织中的表达。切片内阳性细胞数≥20%为ERβ阳性。结果ERβ在肺癌组织中的表达主要定位于细胞核,其表达率为48.4%(31/64),而在癌旁肺组织和肺良性病变组织中无明显表达。ERβ在鳞癌、腺癌中阳性表达率分别为28.9%(11/38),76.9%(20/26),腺癌组织中ERβ表达率显著高于鳞癌(P=0.000 2)。吸烟组ERβ阳性表达率(67.6%,25/37)显著高于非吸烟组(22.2%,6/27)(P=0.000 5),ERβ阳性表达组外周血雌二醇水平(nmol/L)显著高于ERβ阴性组(225.7±75.2 vs 159.5±54.8,P=0.000 1)。结论新型ERβ在肺腺癌和鳞癌中过表达。ERβ与肺癌尤其是肺腺癌的发生可能有关联。吸烟可能与ERβ过表达相关联。肺癌组织ERβ过表达与外周血雌二醇水平增高相一致,肺癌可能分泌雌激素。
Objective To study the expression of estrogen receptor β (ERβ) in lung adenocarcinoma and squamous cell carcinoma, and to explore its relationship with clinicopathological features, smoking and estrogen levels in peripheral blood. Methods Immunohistochemistry and Western blotting were used to detect the expression of ERβ in 26 cases of lung adenocarcinoma and 38 cases of lung squamous cell carcinoma (a total of 37 cases of paracancer tissues) and 15 cases of benign lung lesions. The number of positive cells in the section≥20% was positive for ERβ. Results The expression of ERβ mainly located in the nucleus and the expression rate of ERβ was 48.4% (31/64) in the lung cancer tissues, but not in the paracancer lung tissues and benign lung lesions. The positive rates of ERβ in squamous cell carcinoma and adenocarcinoma were 28.9% (11/38) and 76.9% (20/26), respectively. The expression of ERβ in adenocarcinoma was significantly higher than that in squamous cell carcinoma (P = 0.000 2). The positive rate of ERβ in smoking group was significantly higher than that in non-smoking group (67.2%, 25/37) (22.2%, 6/27) (P = 0.0005) (225.7 ± 75.2 vs 159.5 ± 54.8, P = 0.000 1). Conclusion The novel ERβ is overexpressed in lung adenocarcinoma and squamous cell carcinoma. ERβ and lung cancer, especially lung adenocarcinoma may be related. Smoking may be associated with ERβ overexpression. Lung cancer ERβ overexpression and peripheral blood estradiol levels are consistent, lung cancer may secrete estrogen.