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目的：观察促性腺激素释放激素（ＬＨＲＨ）类似物那法瑞林对体外培养的人乳腺癌细胞ＢＣａＰ－３７生长的影响。方法：培养的ＢＣａＰ－３７细胞，经下列条件作用４ｄ后进行细胞计数：（１）加入１０－５～１０－９ｍｏｌ／Ｌ的那法瑞林；（２）加入１０－７～１０－１０ｍｏｌ／Ｌ的雌二醇（Ｅ２）；（３）在含１０－８ｍｏｌ／ＬＥ２的条件下，加入１０－６～１０－８ｍｏｌ／Ｌ的那法瑞林或他莫昔芬；（４）加入１０ｎｇ／ｍｌ或５０ｎｇ／ｍｌ的表皮生长因子（ＥＧＦ）；（５）在含１０ｎｇ／ｍｌＥＧＦ的条件下，加入１０－５～１０－７ｍｏｌ／Ｌ的那法瑞林。结果：那法瑞林在１０－５～１０－９ｍｏｌ／Ｌ浓度下对ＢＣａＰ－３７培养细胞的生长无显著抑制作用，在１０－６～１０－８ｍｏｌ／Ｌ浓度下可拮抗１０－８ｍｏｌ／ＬＥ２对ＢＣａＰ－３７细胞的生长促进作用，对１０ｎｇ／ｍｌＥＧＦ抑制ＢＣａＰ－３７细胞生长的作用无显著影响。他莫昔芬拮抗Ｅ２促进ＢＣａＰ－３７生长的作用与那法瑞林相似。结论：那法瑞林对人乳腺癌细胞ＢＣａＰ－３７体外培养生长无显著抑制作用，但能拮抗Ｅ２对ＢＣａＰ－３７细胞的生长促进作用
Objective: To observe the effect of gonadotropin-releasing hormone (LHRH) analogue nafarelin on the growth of human breast cancer cell BCaP-37 in vitro. METHODS: Cultured BCaP-37 cells were subjected to cell counting after 4 days of action under the following conditions: (1) adding 10-5 to 10-9 mol/L of nafarelin; (2) adding 10-7 to 10-10 mol/L L estradiol (E2); (3) add 10-6 to 10-8 mol/L of nafarelin or tamoxifen at 10-8 mol/LE2; (4) add 10 ng/L Ml or 50 ng/ml of epidermal growth factor (EGF); (5) Add 10 to 10 to 10-7 mol/L nafarelin in the presence of 10 ng/ml EGF. RESULTS: Nafarelin showed no significant inhibitory effect on the growth of BCaP-37 cultured cells at the concentration of 10-5 to 10-9 mol/L, but it antagonized 10-8 mol/LE2 at the concentration of 10-6 to 10-8 mol/L. The growth promoting effect of BCaP-37 cells had no significant effect on the effect of 10 ng/ml EGF on inhibiting the growth of BCaP-37 cells. Tamoxifen antagonized the effect of E2 on the growth of BCaP-37 similar to that of nafarelin. Conclusion: Nafarelin has no significant inhibitory effect on the growth of human breast cancer cell BCaP-37 in vitro, but it can antagonize the growth promoting effect of E2 on BCaP-37 cells.