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为控制黄曲霉毒素主要产生菌寄生曲霉AS3.4407在粮食储藏环境中的产毒污染,采用组合实验设计策略,考察了五个模拟环境因子对菌株产毒的影响。首先,利用Plack-ett-Burman试验筛选出三个对毒素产量影响显著的因子(转速、温度、pH),在此基础上,采用CCD实验设计法(Central Composite Design,CCD)研究了这三个因子对寄生曲霉AS3.4407产毒影响的交互作用,并根据毒素曲面方程和二次多项回归方程得到该菌的最低产毒条件。在环境通风良好(转速240rpm),储藏温度较低(18℃),pH为弱碱性(pH 8)条件下,寄生曲霉AS3.4407产毒量最低,验证试验证实了该方程的预测值与实际值之间具有较好的拟合度。为粮库采取相应措施,改变储藏环境条件以降低毒素污染提供了很好依据。
In order to control the production toxicity of Aspergillus parasiticus AS3.4407, a major producing strain of Aspergillus toxin, in food storage environment, a combinatorial experimental design strategy was adopted to investigate the effects of five simulated environmental factors on the toxigenicity of the strain. First of all, three factors (speed, temperature, pH) that had a significant effect on toxin production were screened by using the Plack-ett-Burman test. Based on this, three composite designs were studied by CCD (Central Composite Design, CCD) Factor interaction of Aspergillus parasiticus AS3.4407 on toxigenicity, and according to the toxoid surface equation and the quadratic multiple regression equation, the minimum producing conditions of the bacteria were obtained. The production of Aspergillus parasiticus AS3.4407 was the lowest, under the conditions of good aeration (speed of 240rpm), low storage temperature (18 ℃) and pH of weak alkaline (pH 8). The validation test confirmed the predictive value of this equation The actual value of a good fit between degrees. The corresponding measures for the grain storage to change the storage environment to provide a good basis to reduce the toxin pollution.