论文部分内容阅读
利用回交群体和多位点扫描分析方法研究了逆境胁迫下的选择牵连效应,找到相应的基因组区段,并分析了这些区段对不同选择条件的响应。结果如下:随机群体标记偏分离率为34.38%(P<0.000 1),经耐旱、耐盐选择后标记偏分离率分别扩大到51.56%和55.88%。说明经过耐旱、耐盐选择后回交导入系群体等位基因偏分离更为严重,群体基因型结构发生变化,表明选择牵连效应的存在。基于牵连效应的选择响应,根据供体等位基因导入频率在耐旱、耐盐选择群体与随机群体中的表现,经卡方分析在0.000 1水平下检测到3个耐旱相关位点(Satt338、Satt640和Sat_108),5个耐盐相关位点(Sat_271、Satt726、Satt640、Satt513和Sat_108)。
Using backcross analysis and multi-site scanning analysis, we studied the selective implicative effect under stress and found the corresponding genomic segments, and analyzed the response of these segments to different selection conditions. The results were as follows: The partial segregation rate of random population was 34.38% (P <0.0001), and the partial segregation rate after drought-tolerant and salt-tolerant selection increased to 51.56% and 55.88% respectively. The results showed that the segregation of the alleles of the backcross inbred lines after drought-tolerant and salt-tolerant selection was more serious and the genotype structure of the population changed, indicating the existence of the selective implicative effect. Based on the selected response of entrainment effect, three drought-tolerance-related sites (Satt338) were detected at the level of 0.000 1 by chi-square analysis according to the frequency of donor allele import in drought-tolerant and salt-tolerant populations and random populations , Satt640 and Sat_108) and five salt-tolerance-related sites (Sat_271, Satt726, Satt640, Satt513 and Sat_108).