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氨吡酮为一强心类药物,其血、尿药浓度可用反相高效液相色谱法(RPHPLC)测定。氨吡酮及内标安替比林在pH8条件下提取至乙酸乙酯中,再用1 mol/L HCl回提净化后,在YWG-C_(18)H_(87)柱上用流动相甲醇-水-N,N,N′,N′-四甲基乙二胺(37:63:0.3V/V)分离,于UV254nm检测。最低检测浓度为0.1 μg/ml。方法回收率为100.5±0.46％。日内和日间变导系数(CV％)分别为3.78％和1.63％(n=5)。样品的内源性杂质和其他抗心血管病药物不干扰测定。家兔按10 mg/kg单剂量静脉给药后,用本法测定氨吡酮的经时药物浓度,C-T曲线符合二室模型,T_(1/2)(β)=1.99±0.20(p=5),尿中原药24h的排泄率为给药量的38.73％±14.55％。
Praziquantel is a cardiac drug, blood, urine concentration can be determined by reverse-phase high performance liquid chromatography (RPHPLC). Antipyrin and internal standard antipyrine were extracted into ethyl acetate at pH8, and then purified with 1 mol / L HCl, and then eluted on YWG-C_ (18) H_ (87) column with mobile phase of methanol - water -N, N, N ’, N’-tetramethylethylenediamine (37: 63: 0.3 V / V) and detected at UV 254 nm. The minimum test concentration is 0.1 μg / ml. The recovery rate was 100.5 ± 0.46%. The intra-day and inter-day CV (CV%) were 3.78% and 1.63%, respectively (n = 5). Samples of endogenous impurities and other anti-cardiovascular drugs do not interfere with the determination. After intravenous administration of 10 mg / kg single dose to rabbits, the drug concentration of amrinone was determined by this method. The CT curve accorded with two-compartment model with T 1/2 (β) = 1.99 ± 0.20 (p = 5), urinary excretion rate of 24 hours for the original dose of 38.73% ± 14.55%.