采用溶胶法制备了介孔碳/纳米金复合材料,并利用透射电子显微镜、扫描电子显微镜和X射线衍射仪进行了表征。将合成的复合材料修饰于玻碳电极表面,用循环伏安法同时测定多巴胺(DA)、抗坏血酸(AA)和尿酸(UA)。在pH 7.2磷酸盐缓冲溶液中,DA、AA和UA的氧化峰得到了很好的分离;和裸电极比,介孔碳/纳米金修饰电极对DA、AA和UA具有良好的电催化作用,DA、AA和UA的氧化峰的峰电流强度分别增加1.7,2.0,12倍,3种物质的浓度分别在0.20~45.8,4.0~792.0,0.06~166.0μmol·L-1范围内与其峰电流强度呈线性关系,检出限(3S/N)分别为0.075,7.5,0.021μmol·L-1。 Mesoporous carbon / nano-gold composite materials were prepared by sol method and characterized by transmission electron microscopy, scanning electron microscopy and X-ray diffractometry. The synthesized composites were modified on the surface of glassy carbon electrode, and simultaneous determination of dopamine (DA), ascorbic acid (AA) and uric acid (UA) by cyclic voltammetry. The oxidation peaks of DA, AA and UA were well separated in pH 7.2 phosphate buffer solution. And the ratio of bare electrode to mesoporous carbon / gold nanoparticles modified electrode had good electrocatalytic activity for DA, AA and UA, The peak current intensities of DA, AA and UA peaks were increased by 1.7, 2.0 and 12 times, respectively. The concentrations of the three substances were in the range of 0.20 ~ 45.8, 4.0 ~ 792.0 and 0.06 ~ 166.0μmol·L-1, The results showed that the detection limits (3S / N) were 0.075, 7.5 and 0.021μmol·L-1, respectively.