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目的探讨采用随机扩增DNA多态性(RAPD)技术对临床新生儿血培养标本中分离的光滑假丝酵母菌进行基因分型;分析新生儿感染菌株的同源性,追踪其感染源与传播途径,从而为新生儿假丝酵母菌感染防治提供依据。方法 15例新生儿血培养标本中分离的菌株均经过法国科马嘉假丝酵母菌显色鉴定培养基和法国生物梅里埃API20CAUX酵母菌鉴定试剂盒进行鉴定、纯化,其后采用RAPD多态性分析,获得菌株的RAPD指纹图,应用NTSYS软件计算出相似系(SAB),并进行聚类分析,比较菌株间的同源性。结果 15株菌株中14株被鉴定为光滑假丝酵母菌,1株为白色假丝酵母菌,且鉴定分离的2~14号的光滑假丝酵母菌相似系数均≥0.8,表明菌株之间基因型相似度较高,具有基因同源性,而第一株菌株与其他13株之间的相似系数均<0.6,不具有基因同源性。结论本研究发现新生儿感染的13株光滑假丝酵母菌具有基因同源性,初步证实了试验分离的光滑假丝酵母菌在住院患儿间进行水平传播,提示了光滑假丝酵母菌易引起新生儿院内交叉感染,预防宜采取综合措施,切断传播途径。
OBJECTIVE: To investigate the genotyping of Candida glabrata isolated from blood samples of clinical neonates by random amplified polymorphic DNA (RAPD) technique. The homology of neonatal infection strains was analyzed and their infection sources and transmission were traced Ways to provide a basis for the prevention and treatment of neonatal Candida infection. Methods All 15 isolates of blood samples from neonates were identified and purified by Candida albicans colorimetric identification medium and French bioMérieux API20CAUX yeast identification kit. Then, RAPD polymorphism The RAPD fingerprints of the strains were obtained. The similarity lines (SABs) were calculated by NTSYS software, and cluster analysis was performed to compare the homology among isolates. Results Fourteen isolates were identified as Candida glabrata and one as Candida albicans, and the similarity coefficients of Candida glabrata number 2-8 were all ≥0.8, which indicated that the isolates The similarity between the first strains and the other 13 strains were all less than 0.6, which indicated that they had no homology. Conclusions This study found that 13 strains of Candida glabrata infected by neonates had gene homology. It was preliminary confirmed that the level of Candida glabrata isolated from the hospitalized children was horizontally transmitted, suggesting that Candida glabrata could easily cause Neonatal hospital cross-infection, prevention should take comprehensive measures to cut off the route of transmission.