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Objective:To observe the changes of coagulation and fibrinolysis function in human um-bilical cord vein endothelial cells(HUVEC)induced by recombinant human tumor necrosis factor-α(rhT-NF-α)and the effect of Naotaifang extract(脑泰方,NTE)on it.Methods:Cultured HUVEC is randomlydivided into six groups:Control group,NTE Control group(Only 2 g/L NTE),rh TNF-αgroup(100μg/L rhTNF-α),and low-dosage,middle-dosage and high-dosage NTE group(100μg/L rhTNF-αand 0.67g/L,2 g/L,6 g/L NTE).The coagulation activity of frozen-dissolved HUVEC,von Willebrand factor(vWF)content in the conditioned medium and tissue-type plasminogen activator(tPA)and plasminogenactivator inhibitor(PAI)activity were to be detected after 24 hrs.Results:Compared with the controlgroup,PAI activity were enhanced,vWF release markedly increased in conditioned medium of TNF-αgroup(P<0.01)and the frozen-dissolved HUVEC markedly shortens the rabbit plasma prothrombintime,and the above changes could be significantly inhibited by the 3 dosages o
Objective: To observe the changes of coagulation and fibrinolysis function in human um-bilical cords endothelial cells (HUVEC)induced by recombinant human tumor necrosis factor-α(rhT-NF-α) and the effect of Naotaifang extract(脑泰方, NTE) on it.Methods:Cultured HUVEC is randomlydivided into six groups:Control group,NTE Control group (Only 2 g/L NTE),rh TNF-α group (100 μg/L rhTNF-α),and low-dosage,middle- Dosage and high-dosage NTE group (100 μg/L rhTNF-αand 0.67 g/L, 2 g/L, 6 g/L NTE). The coagulation activity of frozen-dissolved HUVEC, von Willebrand factor (vWF) content in the conditioned After medium and tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor (PAI) activity were to be detected after 24 hrs.Results:Compared with the controlgroup, PAI activity was enhanced, vWF release markedly increased in conditioned medium of TNF-α group (P <0.01) and the frozen-dissolved HUVEC markedly shortens the rabbit plasma prothrombintime, and the above changes could be significantly inhibited Ed by the 3 dosages o