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为阐明小瓜虫的形态发生及其对鳃组织的危害过程,本文开展了多子小瓜虫的光镜观察和翘嘴鲌鳃组织病理切片方面的研究。光镜形态研究发现小瓜虫运动时呈球形,直径500—800μm;前端具有耳廓状胞口和内陷的袋状胞咽;环境温度为18℃时,滋养体经1h左右发育成包囊,体表形成一层透明胶膜,并以虫体中央的分裂沟为界进行二分裂;子代虫体从8细胞期进入16细胞期时,包囊内部形成内胞膜将子代虫体等分为两个集团。组织切片结果显示虫体呈卵圆形,挤压了周围的包裹细胞,导致组织变形;鳃小片黏连,鳃丝上皮细胞受到破坏,严重区域鳃丝结构被完全破坏。本研究解析了小瓜虫的形态发生与发育过程以及对宿主危害的组织病理结构与机制,为揭示小瓜虫的侵染、发育和成熟规律奠定了理论基础,并为小瓜虫病害的有效防控提供理论资料。
In order to elucidate the morphogenesis of the small insects and their damage to the gill tissue, this paper carried out the light microscope observation of more than small insects melon and gill histopathology study. Light microscopy morphological studies found that the small melon insects were spherical movement, diameter 500-800μm; front end with auricle-shaped cells and invaginated pouchy pharynx; ambient temperature of 18 ℃, the trophozoites developed into cysts about 1h , The body surface to form a layer of transparent plastic film, and to the body of the dividing fissure ditch bifurcation; offspring from the 8-cell stage into the 16-cell period, the cyst within the cytoplasm will be offspring parasites Divided into two groups. Tissue sections showed that the parasites were oval in shape, squeezing the surrounding wrapping cells, resulting in the deformation of the tissue. The gill lamina adhered to the damaged gill filaments and the gill filaments in the severe area were completely destroyed. In this study, we analyzed the morphogenesis and development of Euphemaceae and the histopathological structure and mechanism of its damage to host, which laid a theoretical foundation for revealing the law of infection, development and maturation of Eupatorium. Prevention and control to provide theoretical information.