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目的构建Rip1-Tag2;P-sel-/-小鼠模型。方法将Rip1-Tag2小鼠与P-选择素敲除(PKO)小鼠杂交,剪尾提DNA后用PCR法检测小鼠的基因型。结果 Rip1-Tag2小鼠与PKO小鼠杂交三代后,Rip1-Tag2;P-sel-/-小鼠模型构建成功。结论将Rip1-Tag2小鼠与PKO小鼠杂交三代后成功建立了Rip1-Tag2;P-sel-/-小鼠模型,并进行繁育。
Objective To construct Rip1-Tag2; P-sel - / - mouse model. Methods The Rip1-Tag2 mice were crossed with P-selectin knockout (PKO) mice. The genotypes of mice were detected by PCR after the tail DNA was extracted. Results The Rip1-Tag2; P-sel - / - mouse model was successfully constructed after three generations of hybridization between Rip1-Tag2 and PKO mice. Conclusion The Rip1-Tag2; P-sel - / - mouse model was successfully established after crossing three generations of Rip1-Tag2 mice and PKO mice and breeding.