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目的:探讨帕金森(PD)大鼠模型体内脑黑质和纹状体内骨形成蛋白4(BMP4)及其mRNA表达的变化规律。方法:用六羟基多巴胺(6-OHDA)建立PD模型后,第2、4、6、8和10周时处死大鼠,取左侧黑质、纹状体,用免疫组化、酶联免疫、荧光定量PCR技术从蛋白水平和基因水平检测BMP4及其mRNA表达。结果:BMP4及其mRNA表达基本一致,其BMP4及mRNA表达均呈双峰,其蛋白表达在纹状体内第2周和6周时达高峰、在黑质内第2周和8周时达高峰,其mRNA表达在纹状体内第2周和8周时达高峰、在黑质内第4周和8周达高峰,差异均有统计学意义(P<0.05)。结论:PD大鼠模型体内,BMP4及其mRNA不呈现稳定的低表达,而是有波动性,明确BMP4及其mRNA处于稳定低表达的时间后,为下一步的治疗实验时间点的选择上奠定了基础。
AIM: To investigate the changes of bone morphogenetic protein 4 (BMP4) and its mRNA expression in brain substantia nigra and striatum in Parkinson’s (PD) rat model. Methods: PD model was established with hexahydroxy-dopamine (6-OHDA). Rats were sacrificed at the 2nd, 4th, 6th, 8th, and 10th week. The left substantia nigra and striatum were removed. Immunohistochemistry Fluorescent quantitative PCR was used to detect the expression of BMP4 and its mRNA from the protein level and the gene level. Results: BMP4 and its mRNA expression were basically the same. The expression of BMP4 and mRNA showed a bimodal peak. The protein expression peaked at the second week and the sixth week in the striatum, and peaked at the second week and the eighth week in the substantia nigra. Reached the peak at week 2 and week 8 in striatum, peaked at week 4 and week 8 in substantia nigra, with statistical significance (P <0.05). CONCLUSION: The expression of BMP4 and its mRNA in PD rat model does not show stable low expression, but fluctuates. It is clear that BMP4 and its mRNA are stable and low expression time, which will lay the foundation for the choice of treatment experiment time point The foundation.