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作者将编码鼠白细胞介素2(IL-2)的互补DNA插入表达流感病毒血凝素(HA)的牛痘病毒重组体VV-HA中,组建成VV-HA-IL2。同时还组建了表达单纯疱疹病毒胸苷激酶(TK)的对照病毒VV-HA-TK。VV-HA-IL2感染人类143B细胞后,4小时内即可在上清液检出大量具有生物学活性的IL-2,12小时左右达最高活性。在无胸腺裸鼠右后足垫接种VV-HA-IL2或VV-HA-TK。VV-HA-IL2引起足部轻微水肿,几天后消失;而VV-HA-TK则引起严重坏死灶,至第30天仍不消退,以后从足部发现高滴度病毒(6×10~5～1.5×10~7蚀斑形成单位)。接种以上两种重组
The authors inserted the complementary DNA encoding murine interleukin 2 (IL-2) into the vaccinia virus recombinant VV-HA expressing the influenza virus hemagglutinin (HA) to form VV-HA-IL2. The control virus VV-HA-TK expressing herpes simplex virus thymidine kinase (TK) was also constructed. After inoculation of human 143B cells with VV-HA-IL2, a large amount of biologically active IL-2 was detected in the supernatant within 4 hours and reached the highest level in about 12 hours. VA-HA-IL2 or VV-HA-TK was vaccinated in athymic nude mice right hind footpad. VV-HA-IL2 caused slight edema in the foot and disappeared in a few days. However, VV-HA-TK caused severe necrosis and did not disappear on the 30th day. Later, high titer virus was found from the foot (6 × 10 ~ 5 ~ 1.5 × 10 ~ 7 plaque forming units). Inoculation of the above two reorganization