我们利用分子生物学技术(组织总RNA的斑点印迹和Northern印迹分析)研究了高脂膳食大鼠肝、肠等脏器中LDL受体mRNA水平的变化以及药理剂量苯甲酸雌二醇(EB,4mg/kg/日)对该mRNA水平的影响。结果发现,高脂组(饲料中含2%胆固醇、5%蛋黄粉、10%猪油及0.2%胆酸钠)肝LDL受体mRNA水平较正常组降低近50%(P<0.05);高脂+EB组该mRNA水平较高脂组升高3.5倍(P<0.002),并超过 We used molecular biology techniques (dot blot and Northern blot analysis of tissue total RNA) to study LDL receptor mRNA levels in liver, intestine and other organs in high fat diet rats and pharmacological doses of estradiol benzoate (EB, 4 mg / kg / day) on this mRNA level. The results showed that the level of LDL receptor mRNA in high fat diet group (feed containing 2% cholesterol, 5% egg yolk powder, 10% lard and 0.2% sodium cholate) was reduced by nearly 50% (P <0.05) Compared with the high-fat group, the mRNA level in lipid + EB group increased by 3.5 times (P <0.002) and exceeded