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目的 观察阳离子脂质体 DOSPER介导或 Bcl- 2反义寡核苷酸 G3139直接作用时 ,造血系统恶性肿瘤细胞株对 G3139的摄入 ,为阳离子脂质体和反义寡核苷酸的进一步应用提供依据。 方法 利用异硫氰酸荧光素标记的 G3139,流式细胞仪 (FCM)测定造血系统恶性肿瘤细胞株 HL6 0、K5 6 2、U937、CA46和 CEM细胞内的平均荧光强度。 结果 G3139直接作用于细胞时 ,细胞内荧光强度 U937>HL6 0 >CA46 >K5 6 2 >CEM(P<0 .0 5 ) ;DOSPER介导转染时 ,各细胞内的荧光强度均显著高于直接转染时 (P<0 .0 1) ,而且当 DOSPER/ G3139(μg/μg )为 2～ 3∶ 1时 ,转染效率最佳 ,但此时各细胞间细胞内平均平均荧光强度无明显的差别 (P>0 .0 5 )。 结论 不同造血系统恶性肿瘤细胞株对 G3139的摄入不同 ,阳离子脂质体 DOSPER可普遍提高细胞对 G3139的摄入。
OBJECTIVE To observe the direct effect of cationic liposomes DOSPER-mediated or Bcl-2 antisense oligonucleotide G3139 on the uptake of G3139 by hematopoietic malignant tumor cell lines for cationic liposomes and antisense oligonucleotides Application basis. Methods Fluorescein isothiocyanate labeled G3139 and flow cytometry (FCM) were used to determine the average fluorescence intensity of HL6 0, K5 6 2, U937, CA46 and CEM cells in hematopoietic malignant tumor cell lines. Results When G3139 was directly administered to cells, the intracellular fluorescence intensity was significantly higher than that of U937> HL6 0> CA46> K5 6 2> CEM (P <0. 05) The transfection efficiency was the best when transfection was performed at the DOSPER / G3139 (μg / μg) of 2 ~ 3: 1, but the intracellular average fluorescence intensity Significant difference (P> 0.05). Conclusions The uptake of G3139 by different hematopoietic malignant tumor cell lines is different. The cationic liposome DOSPER can generally increase the cell uptake of G3139.