论文部分内容阅读
用Northern blot方法对二乙基亚硝胺所诱发的大鼠肝癌中内源性蛋白酶抑制因子α_2-巨球蛋白(α_2-M)、非特异性免疫抑制剂α_1-酸性糖蛋白(α_1-AGP)及雄性激素正调控的α-2u球蛋白(α-2u)三种分泌性蛋白基因表达情况进行了分析。结果表明在大部分(14/16)肝癌样品中α_2-M RNA水平显著降低;而α_1-AGP RNA水平显著高于正常对照水平;α-2u RNA水平明显下降,但在某些雄性大鼠肝癌样品中该基因却有一定程度的表达。这些结果说明,一些肿瘤宿主血浆中α_2-M水平的显著下降及α_1-AGP水平的明显升高分别是由于基因表达活性的下降及升高所致。α-2u基因表达的异常提示,在癌变过程中机体的内分泌功能发生了某些变化。
Northern blot was used to detect endogenous protease inhibitor α_2-macroglobulin (α_2-M), α_1 -AGP (α_1 -AGP) in rat hepatocellular carcinoma induced by diethylnitrosamine And α-2u globulin (α-2u), which are positively regulated by androgen, were analyzed. The results showed that the level of α_2-M RNA was significantly decreased in the majority of (14/16) liver cancer samples; the level of α_1-AGP RNA was significantly higher than that of the normal control; the level of α-2u RNA was significantly decreased; however, in some male rats, The sample has a certain degree of expression of the gene. These results suggest that the significant decrease of α_2-M level in plasma of some tumor hosts and the significant increase of α_1-AGP level are due to the decrease and increase of gene expression activity, respectively. The abnormal expression of α-2u gene suggests that some changes have taken place in the endocrine function of the body during carcinogenesis.