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从草鱼养殖水体中分离培养水霉菌,以天然营养物直接作为培养基质有较好的效果。其中玉米片诱生水霉菌丝所需时间最短,但菌丝崩解速度也较快;油菜籽诱生菌丝所需时间稍长,但菌丝长势较一致且崩解较慢,利于后续研究。游动孢子囊的诱生仍以油菜籽为佳,有性孢子的诱生以油菜籽和玉米片相结合为好。游动孢子囊的最适诱生温度为25℃,而与有性繁殖有关的藏卵器和精子器的最适诱生温度为15℃。
Isolation and culture of water fungus from the grass carp breeding water to natural nutrients as a direct substrate culture have good results. Among them, the time for inducing M. mildew in corn flakes was the shortest, but the mycelial disintegration rate was faster. The time for inducing hyphae in rapeseed was longer, but the growth of hyphae was more consistent and the disintegration was slower, which was benefit to follow-up study . The zoospore is still induced by rapeseed, and the induction of zoospore is combined with rapeseed and cornflakes. The most suitable temperature for zoospore bursting is 25 ℃, while the optimum temperature for the oviparous and sperm-related reproductive plants is 15 ℃.