High-pressure (HP) or ultrahigh-pressure (UHP) rutile-quartz veins that form at mantle depths due to fluid-rock interaction can be used to trace the properties and behavior of natural fluids in subduction zones. To explore the fluid flow and the associated element mobility during deep subduction and exhumation of the continental crust, we investigated the major and trace elements of Ti-rich minerals. Additionally, U–Pb dating, trace element contents, and Lu–Hf isotopic composition of zircon grains in the UHP eclogite and associated rutile-quartz veins were examined in the North Qaidam UHP metamorphic belt, Yuka terrane. The zircon grains in the rutile-quartz veins have unzoned or weak oscillatory zonings, and show low Th/U ratios, steep chondrite-normalized patts of heavy rare earth elements (HREEs), and insignificant negative Eu anomalies, indicating their growth in metamorphic fluids. These zircon grains formed in 431 ± 3 Ma, which is consistent with the 432 ± 2 Ma age of the host eclogite. As for the zircons in the rutile-quartz veins, they showed steep HREE patts on one hand, and were different from the zircons present in the host eclogite on the other. This demonstrates that their formation might have been related to the breakdown of the early stage of get, which corresponds to the abundance of fluids during the early exhumation stage. The core-rim profile analyses of rutile recorded a two-stage rutile growth across a large rutile grain;the rutile core has higher Nb, Ta, W, and Zr contents and lower Nb/Ta ratios than the rim, indicating that the rutile domains grew in different metamorphic fluids from the core towards the rim. The significant enrichment of high field strength elements (HFSEs) in the rutile core suggests that the peak fluids have high solubility and transportation capacity of these HFSEs. Furthermore, variations in the Nb vs. Cr trends in rutile indicate a connection of rutile to mafic protolith. The zircon grains from both the rutile-quartz veins and the host eclogite have similar Hf isotopic compositions, indicating that the vein-forming fluids are intally derived from the host eclogite. These fluids accumulated in the subduction channel and were triggered by local dehydration of the deeply subducted eclogite during the early exhumation conditions.