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目的:研究吗啡对不同淋巴细胞增殖的作用及纳洛酮的影响.方法:观察吗啡对未成熟的、静止的及活化的脾脏淋巴细胞体外增殖影响及纳洛酮的阻断作用.结果:吗啡(1×10~(-10)—1×10~(-6)mol L~(-1))能增加Con A诱导的T-细胞的增殖,1 μmol L~(-1)还能促进LPS诱导的B-细胞的增殖,同时这些增强作用都能被纳洛酮50μmol L~(-1)阻断,纳洛酮单独亦能促进活化T-细胞的增殖.而吗啡1×10~(-10)—1×10~(-5)mol L~(-1)对静止的脾脏淋巴细胞及Con A活化的胸腺淋巴细胞的增殖都无影响.但是吗啡1mmol L~(-1)能广泛抑制静止的、LPS活化的脾脏细胞及Con A活化的胸腺,脾脏淋巴细胞增殖,且都不能被纳洛酮阻断.结论:吗啡对活化T-和B-细胞的促进作用是由细胞表面的阿片受体介导的,此阿片受体随着淋巴细胞的成熟和活化而变化,而吗啡1 mmol L~(-1)对淋巴细胞增殖的抑制作用却不是由经典的阿片受体介导的.
Objective: To study the effect of morphine on the proliferation of different lymphocytes and the effect of naloxone.Methods: Morphine was used to observe the effect of morphine on the proliferation of immature, immortal and activated spleen lymphocytes and the blocking effect of naloxone.Results: Morphine (1 × 10 ~ (-10) -1 × 10 ~ (-6) mol L ~ (-1)) could increase Con A-induced proliferation of T-cells, and 1 μmol L -1 could also promote LPS Induced proliferation of B-cells, meanwhile these potentiation were all blocked by naloxone 50μmol L -1, naloxone could also promote the proliferation of activated T-cells alone.While morphine 1 × 10 ~ (-1) 10) -1 × 10 -5 mol L -1 had no effect on the proliferation of splenic lymphocytes and Con A-activated thymocytes, but morphine 1 mmol L -1 could be widely inhibited Resting, LPS-activated spleen cells and Con A-activated thymus and spleen lymphocytes proliferated, and none of them were blocked by naloxone.CONCLUSION: The promotion of morphine on activated T- and B-cells is mediated by opium at the cell surface Receptor-mediated opioid receptor changes with the maturation and activation of lymphocytes, whereas the inhibitory effect of morphine 1 mmol L -1 on lymphocyte proliferation was not mediated by classical opioid receptors.