用[~3H]Dex作为配体,证实小鼠游离脾细胞有[~3H]Dex特异结合部位,该部位有低容量(表观结合容量为10.70±2.35 fmol/10~6个细胞)、高亲和力(表观平衡解离常数为6.37±1.97 nM)、甾体结合特异性等特点,具备了作为GCR的基本判据。用CYCLO抑制蛋白合成的方法,测定了该GCR的半衰期。在蛋白质合成被抑制70%的情况下,GCR的半衰期为1.6±0.5h,降解速度常数为0.455±0.132h~(-1),表明GCR是代谢活跃的蛋白质,提示GCR不仅是激素信息的接受者和传递者,而且可能是激素反应的调节者。 Using [~ 3H] Dex as a ligand, [~ 3H] Dex specific binding sites of mouse free splenocytes were confirmed, showing low capacity (apparent binding capacity of 10.70 ± 2.35 fmol / 10-6 cells) and high Affinity (apparent equilibrium dissociation constant of 6.37 ± 1.97 nM), steroid binding specificity and other characteristics, with the basic criteria for GCR. The half-life of this GCR was determined by the method of CYCLO inhibition of protein synthesis. The GCR had a half-life of 1.6 ± 0.5h and a degradation rate constant of 0.455 ± 0.132h ~ (-1), indicating that GCR is a metabolically active protein, suggesting that GCR is not only an acceptor of hormonal information And deliverer, and may be regulators of hormonal responses.