论文部分内容阅读
利用Giemsa染色技术对柑桔不同种和品种的愈伤组织及再生的多丛芽进行了染色体检查。结果表明,柑桔愈伤组织中染色体变异是一普遍现象。从锦橙申分离出A、B、C三种类型的愈伤组织,其中A类变异最大,含有0.4%的单倍体、35.1%的二倍体、3.8%的三倍体、49.1%的四倍体、1.3%的六倍体、0.4%的八倍体、0.09%的十倍体和0.03%的十六倍体细胞以及9.9%的非整倍体细胞,C类变异最小,B类介于A、C两类之间。A类再生出的多丛芽中,有16.5%的三倍体和34.5%的四倍体。在生根培养基中加入0.4%的活性炭,能显著地促进多丛芽的增殖和生长。2,4-D对愈伤组织染色体变异有促进作用。
Giemsa staining was used to examine the callus and regenerated shoots of different species and varieties of citrus. The results show that the chromosomal variation in citrus callus is a common phenomenon. A, B and C callus were isolated from Jin-Orange, among them, the largest variation in type A was 0.4% haploid, 35.1% diploid, 3.8% triploid, 49.1% Tetraploid, 1.3% hexaploid, 0.4% octaploid, 0.09% hexaploid, 0.03% hexaploid and 9.9% aneuploid cells. Between A, C between the two categories. In the A-type reproductive shoots, there are 16.5% triploids and 34.5% tetraploids. Adding 0.4% activated carbon into the rooting medium can significantly promote the proliferation and growth of many buds. 2,4-D on callus chromosome promotion role.