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目的:观察比较活血化瘀方、清热祛湿方和养阴柔肝方3种不同治法的中药复方对H22肝癌移植瘤抑瘤作用,并初步探讨其机理。方法:采用皮下接种肝癌H22细胞的方法建立荷瘤小鼠模型,分别用上述3种中药复方水煎液ig给药11 d,观察各组荷瘤小鼠的肿瘤生长情况,采用缺口末端标记法测定肿瘤细胞凋亡指数,免疫组织化学方法测定凋亡蛋白Bcl-2,Bax的表达情况。结果:与模型对照组比较,3种复方均可抑制H22肝癌移植瘤生长,促进瘤体细胞凋亡,降低瘤体细胞Bcl-2/Bax比值(P<0.01);其中清热祛湿方与养阴柔肝方作用后Bcl-2/Bax分别为0.16±0.04,0.19±0.02,显著低于模型组(2.71±0.06),且与活血化瘀方1.24±0.10比较差异具有统计学意义(P<0.01),但前二者之间比较无统计学意义(P>0.05)。结论:活血化瘀、清热祛湿、养肝柔肝3种方法均可抑制H22肝癌移植瘤生长,其机制与通过调控凋亡相关蛋白Bcl-2,Bax的表达诱导肿瘤细胞凋亡有关。
OBJECTIVE: To observe and compare the antitumor effects of three traditional Chinese medicine prescriptions of Huoxue Huayu Recipe, Qingre Qushi Decoction and Yangyin Rougan Recipe on H22 hepatoma transplanted tumor, and to explore its mechanism. Methods: The tumor-bearing mice model was established by subcutaneous inoculation of hepatocellular carcinoma H22 cells. Each of the three kinds of compound decoctions were dosed with ig for 11 days to observe the tumor growth of tumor-bearing mice in each group. The apoptotic index of tumor cells was determined, and the expression of Bcl-2 and Bax proteins were detected by immunohistochemistry. Results: Compared with the model control group, all the three compounds could inhibit the growth of H22 hepatocarcinoma xenografts, promote the apoptosis of tumor cells and decrease the ratio of Bcl-2 / Bax in tumor cells (P <0.01) Bcl-2 / Bax was significantly lower (0.16 ± 0.04,0.19 ± 0.02) than that of model group (2.71 ± 0.06), and the difference was statistically significant (P <0.01) ), But the former two had no statistical significance (P> 0.05). CONCLUSION: Three kinds of methods of promoting blood circulation to remove blood stasis, removing dampness and dampness, and nourishing liver and activating liver can all inhibit the growth of H22 hepatocellular carcinoma xenografts. The mechanism is related to the apoptosis of tumor cells through regulating the expressions of Bcl-2 and Bax proteins.