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目的研究稀土氧化钕暴露对大鼠肺泡巨噬细胞(NR8383)的毒性及其炎性因子分泌的影响。方法将处于对数生长期的NR8383细胞,暴露于终浓度分别为0(对照,PBS)、12.5、25、50、100、200μg/ml的氧化钕混悬液12、24、48 h后,采用MTT法测定NR8383细胞的抑制率;终浓度为0(对照,PBS)、25、50、100μg/ml的氧化钕混悬液24 h后,采用酶标法测定细胞培养液上清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)及白介素-8(IL-8)的浓度。结果与对照组比较,各浓度氧化钕暴露不同时间后NR8383细胞的抑制率均较高,各浓度氧化钕暴露NR8383细胞上清中TNF-α、IL-1β、IL-8的浓度均较高,差异有统计学意义(P<0.05);且随着氧化钕暴露浓度的升高,NR8383细胞的抑制率及上清中TNF-α、IL-1β、IL-8的浓度均呈上升趋势。氧化钕暴露NR8383细胞的抑制率与上清中TNF-α、IL-1β、IL-8的浓度均呈正相关(P<0.01);且NR8383细胞上清中TNF-α、IL-1β、IL-8浓度两两之间均呈正相关(P<0.01)。结论氧化钕刺激可引起NR8383细胞分泌炎性因子增加。
Objective To study the effects of rare earth neodymium oxide on the toxicity and secretion of inflammatory cytokines in rat alveolar macrophages (NR8383). Methods NR8383 cells in logarithmic growth phase were exposed to neodymium oxide suspensions at concentrations of 0 (control, PBS), 12.5, 25, 50, 100 and 200 μg / ml for 12, 24, The inhibitory rate of NR8383 cells was determined by MTT assay. The final concentrations of 0 (control, PBS), 25,50,100μg / ml neodymium oxide suspension were measured 24 h by enzyme-linked immunosorbent assay -α (TNF-α), interleukin-1β (IL-1β) and interleukin-8 (IL-8) Results Compared with the control group, the inhibitory rates of NR8383 cells were higher at various concentrations of neodymium exposure for a long time. The concentrations of TNF-α, IL-1β and IL-8 in NR8383 cells exposed to various concentrations of neodymium oxide were higher, (P <0.05). With the increase of neodymium oxide concentration, the inhibitory rate of NR8383 cells and the concentrations of TNF-α, IL-1β and IL-8 in the supernatant all showed an increasing trend. Neodymium neodymium exposed NR8383 cells inhibition rate and the supernatant of TNF-α, IL-1β, IL-8 concentrations were positively correlated (P lt; 0.01); and NR8383 cell supernatant TNF-α, IL- 8 concentrations between the two were positively correlated (P <0.01). Conclusion Neodymium oxide can induce the secretion of inflammatory cytokines in NR8383 cells.