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目的:研究紫草药材的高效液相色谱(HPLC)指纹图谱,并测定其中β-乙酰氧基异戊酰阿卡宁的含量。方法:色谱柱为ACE C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸溶液(70∶30,V/V),检测波长为516 nm,流速为1.0 ml/min。结果:所得紫草药材的HPLC指纹图谱分离度、精密度、重复性和稳定性均良好;10批紫草药材指纹图谱的相似度均>0.950。β-乙酰氧基异戊酰阿卡宁的进样量在0.3~9.0μg范围内与其峰面积积分值呈良好的线性关系(r=0.999 9);平均加样回收率为101.40%,RSD=4.08%(n=6);不同产地紫草药材中β-乙酰氧基异戊酰阿卡宁的含量差异较大。结论:β-乙酰氧基异戊酰阿卡宁可作为紫草药材含量测定和指纹图谱研究的指标性成分。所建方法可以为2015年版《中国药典》紫草药材标准的修订提供一定的依据。
Objective: To study HPLC fingerprinting of Lithospermum officinale, and determine the contents of β-acetoxyisovaleryl aconitine in it. METHODS: The chromatographic column was ACE C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% formic acid solution (70:30, V / V) at a detection wavelength of 516 nm and a flow rate of 1.0 ml / min. Results: The HPLC fingerprints of the obtained Lithospermum officinale had good resolution, precision, repeatability and stability. The similarity of fingerprints of 10 medicinal herbs was> 0.950. The calibration curve of β-acetoxy isopentyl accanine showed a good linear relationship with the peak area integral value in the range of 0.3-9.0 μg (r = 0.999 9). The average recoveries were 101.40%, RSD = 4.08% (n = 6). The contents of β-acetoxyisovaleryl aconitine in the different origins of Lithospermum officinale were significantly different. Conclusion: β-Acetoxyisovaleryl aconitine can be used as an index component in the determination of medicinal materials and fingerprints of Lithospermum. The proposed method can provide a basis for the revision of the standard of Chinese Pharmacopoeia of Lithospermum in 2015 edition.