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目的:观察ADPKD小型猪肾脏组织中细胞增殖和凋亡的改变。方法:使用PKD1基因敲除的小型猪模型,用Western blot和免疫组化方法检测肾脏组织中细胞增殖指标PCNA;增殖相关的mTOR信号通路分子(phospho-mTOR、phospho-p70S6、phospho-4EBP1)和ERK信号通路分子(phospho-PKA、phospho-MEK、phospho-ERK)的表达水平以及内皮细胞粘附分子CD31以及凋亡相关蛋白Bcl-2、Bax、caspase 3的表达变化。结果:在ADPKD小型猪肾脏组织中,增殖指标PCNA表达显著升高。mTOR信号通路分子phospho-mTOR、phospho-p70S6、phospho-4EBP1水平明显升高,ERK信号通路分子中phospho-PKA、phospho-MEK、phospho-ERK水平明显升高。CD31表达明显升高,Bax/Bcl-2的比值以及caspase 3的表达水平显著升高。结论:本研究显示在ADPKD小型猪肾脏组织中,细胞增殖和凋亡信号通路明显激活。
Objective: To observe the changes of cell proliferation and apoptosis in kidneys of ADPKD miniature pigs. Methods: The model of PKD1 knockout piglets was used to detect the expression of proliferating cell nuclear antigen (PCNA) and phosphorylation of phosphorylated mTOR (phospho-p70S6, phospho-4EBP1) in renal tissues by Western blot and immunohistochemistry The expression of phospho-PKA, phospho-ERK and the expression of CD31 and Bcl-2, Bax and caspase 3 in endothelial cells. Results: In ADPKD miniature pig kidney, the proliferation index PCNA expression was significantly increased. The levels of phospho-mTOR, phospho-p70S6 and phospho-4EBP1 in mTOR signaling pathway were significantly increased, and the levels of phospho-PKA, phospho-MEK and phospho-ERK in ERK signaling pathway were significantly increased. CD31 expression was significantly increased, Bax / Bcl-2 ratio and caspase 3 expression was significantly increased. Conclusion: This study shows that in ADPKD miniature pig kidney tissue, cell proliferation and apoptosis signaling pathway was significantly activated.