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目的了解婴儿毛细支气管炎的病原分布,鼻病毒(RV)感染状况及RV感染所致婴儿毛细支气管炎的临床特征。方法选择毛细支气管炎患儿187例。采用半巢式PCR法检测患儿鼻咽分泌物RV RNA;采用免疫荧光方法测定流感病毒A、流感病毒B、呼吸道合胞病毒(RSV)、腺病毒,及副流感病毒1、2、3型感染情况。结果 187例毛细支气管炎患儿中,RV感染占31.55%,RSV感染占22.46%。RV感染患儿与RSV感染患儿性别、月龄、起病天数和Lowell评分比较差异均无统计学意义(Pa>0.05),缓解天数比较差异有统计学意义(P=0.050),RV感染患儿较RSV感染患儿临床症状缓解快。结论 RV是引起婴儿毛细支气管炎的最常见病原体,RV感染患儿较RSV感染患儿缓解迅速。半巢式PCR方法检测患儿鼻咽分泌物鼻病毒RNA具有很高的敏感性、特异性。
Objective To understand the pathogen distribution of infant bronchiolitis, the status of rhinovirus (RV) infection and the clinical features of infant bronchiolitis due to RV infection. Methods 187 children with bronchiolitis were selected. Detection of nasopharyngeal secretions RV RNA in children with semi-nested PCR; Flu virus A, influenza virus B, respiratory syncytial virus (RSV), adenovirus, and parainfluenza virus type 1, 2, and 3 Infection. Results 187 children with bronchiolitis, RV infection accounted for 31.55%, RSV infection accounted for 22.46%. There was no significant difference in gender, age, onset time and Lowell score between children with RV infection and RSV infection (Pa> 0.05), and there was significant difference between the number of days of remission (P = 0.050), RV infection Children than RSV infection in children with clinical symptoms relieved quickly. Conclusion RV is the most common causative agent of infantile bronchiolitis. Children with RV infection have a better response than those with RSV infection. Semi-nested PCR detection of nasopharyngeal rhinovirus rhinovirus RNA in children with high sensitivity and specificity.