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2007年底在江苏省兴化市保护地栽培番茄上发生了一种危害异常严重的病毒病,其病原初步鉴定为粉虱传双生病毒(Whitefly-transmitted geminiirus,WTG),为明确其在江苏省的发生情况及危害程度,2008年作者在省内各地展开调查,共采集病样115份并对其进行了分子鉴定。检测结果表明:2008年该病在南京、苏州、常州、徐州、泰州和盐城等地均有发生,已遍布于苏南、苏中、苏北;与2007年的发生情况相比,呈明显的蔓延态势。对其中17份代表性分离物的部分序列进行了序列测定,序列分析结果显示这些分离物的序列之间有着98.2%～100.0%的同源性,BLAST分析显示其与番茄黄化曲叶病毒(Tomato yellow leaf curl uirus,TYLCV)同源性最高(超过99.0%)。
At the end of 2007, a serious anomaly virus disease occurred on the cultivated tomato in Xinghua, Jiangsu Province. The pathogen was initially identified as Whitefly-transmitted geminiirus (WTG) The occurrence and the degree of harm, in 2008 the author conducted a survey in various parts of the province, a total of 115 pathological samples were collected and its molecular identification. The test results showed that: in 2008 the disease occurred in Nanjing, Suzhou, Changzhou, Xuzhou, Taizhou and Yancheng and other places have occurred in southern Jiangsu, central Soviet Union and northern Jiangsu; compared with the incidence in 2007, was significantly Spread the situation. The partial sequences of 17 representative isolates were sequenced. Sequence analysis showed that there was a 98.2% ~ 100.0% homology between the sequences of these isolates. BLAST analysis showed that these isolates shared the same sequence as Tomato yellow leaf curl virus ( Tomato yellow leaf curl uirus (TYLCV) had the highest homology (more than 99.0%).