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使用电化学阻抗法(EIS)和循环伏安法(CV)研究了野生型八肋游仆虫中心蛋白(EoCen)在玻碳电极表面的吸附特性及EoCen与Eu3+的相互作用过程。研究发现EoCen在电极表面的吸附不受电极电势的影响;EoCen与N端中心蛋白(N-EoCen)的吸附性相比,吸附速率相近,吸附自由能相近。CV和EIS滴定实验都证明Eu3+与EoCen形成了配位比为4∶1的配合物。并且滴定曲线能够区分EoCen上的四个金属离子结合位点,与使用光谱方法不能有效区分这四个位点相比,电化学方法更具优势。
The adsorption properties of EoCen on the surface of glassy carbon electrode and the interaction between EoCen and Eu3 + were studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). It was found that the adsorption of EoCen on the electrode surface was not affected by the electrode potential. The adsorption rate of EoCen was similar to that of the N-terminal center protein (N-EoCen) and the adsorption free energy was similar. Both CV and EIS titration experiments show that Eu3 + forms a 4: 1 coordination complex with EoCen. And the titration curve distinguishes four metal ion binding sites on EoCen, the electrochemical approach is more advantageous than using spectral methods to effectively distinguish these four sites.