白菜感染芜菁花叶病毒或菸花叶病毒的油菜毒株后叶内和根内游离氨基酸的比较

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感染芜菁花叶病毒的白菜病叶在病害严重后期常出现精氨酸,感染菸花叶病毒的油菜毒株(YMV_(15))的病叶却与健叶相同。而感染以上两病毒的白菜根内,游离氨基酸或酰胺的种类都没有变化。接种后6—8天,感染芜菁花叶病毒的白菜病叶内的天门冬氨酸、谷氨酸、谷酰胺、丝氨酸和苏氨酸的含量有增加,其中尤以天门冬氨酸和丝氨酸增加较为显著;白菜病根内的谷酰胺、谷氨酸、天门冬氨酸、苏氨酸的含量也有上升,这与叶感染病毒的反应一致;而感染 YMV_(15)的病叶内在接种后3—4天,天门冬氨酸和谷氨酸仅稍有增加,白菜病根内在接种后第15天,谷酰胺和丝氨酸的含量才有显著增高。但所有这些游离氨基酸或酰胺含量的增高都是暂时的,随着植物的生长其含量又逐步接近于健株。无论感染芜菁花叶病毒或 YMV_(15)的白菜根,在病害严重后期,组氨酸显著增多,这是与叶反应不同之点。 The cabbage leaf infected with turnip mosaic virus often appears arginine in the late stage of severe disease, while the leaf of the infected canola strain (YMV_ (15)) is the same as the healthy leaf. The cabbage root infected with the above two viruses, the type of free amino acids or amides have not changed. 6-8 days after inoculation, the contents of aspartate, glutamate, glutamine, serine and threonine in cabbage leaf infected with turnip mosaic virus increased, especially aspartic acid and serine The content of glutamine, aspartate and threonine in cabbage root also increased, which was consistent with the response of leaf-infected virus. In the infected leaves infected with YMV_ (15) -4 days, aspartic acid and glutamic acid increased only slightly, the content of glutamine and serine in cabbage root increased significantly on the 15th day after inoculation. However, the increase of all these free amino acids or amides is temporary, and as the plant grows, its content gradually approaches that of healthy plants. Whether inoculated with turnip mosaic virus or YMV_ (15), the content of histidine increased significantly during the later stages of disease, which is different from leaf reaction.
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