从实验室的5株有机磷细菌中筛选到1株解磷能力强的菌株PSB-2。通过L9(34)正交设计研究其最优培养条件,添加不同金属离子对解磷能力的影响,置换培养液的卵磷脂对用2种有机磷农药的降解能力。结果表明:pH值对解磷能力的影响最大,菌PSB-2在最优培养条件下发酵液中有效磷含量可以达到9.610 mg/100g。最优培养条件是:葡萄糖15 g/L,硫酸铵1.0 g/L,pH 6.0,培养60 h。金属离子Mg2+、Ca2+、Na+、Mn2+能够显著提高解磷能力,而Al3+、Fe2+对菌体的解磷能力有明显抑制作用(P<0.05)。PSB-2菌对有机磷农药保棉丰有一定的降解能力。 A strain of strain PSB-2 with high phosphate-solubilizing ability was screened from 5 organophosphorus bacteria in laboratory. The optimal culture conditions of L9 (34) orthogonal design were studied. The effects of different metal ions on the ability of phosphate solubilizing and the degradation ability of lecithin with two kinds of organophosphorus pesticides were studied. The results showed that pH value had the greatest effect on the capacity of phosphate solubilization. The content of available phosphorus in fermentation broth of strain PSB-2 could reach 9.610 mg / 100g under the optimal culture conditions. The optimal culture conditions were glucose 15 g / L, ammonium sulfate 1.0 g / L, pH 6.0 and culture for 60 h. Metal ions such as Mg2 +, Ca2 +, Na + and Mn2 + could significantly improve the ability of phosphate solubilization, while Al3 + and Fe2 + significantly inhibited the ability of phosphate solubilization (P <0.05). PSB-2 bacteria on organic phosphorus pesticide cotton abundance have a certain degree of degradation.