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目的在机体,铁调素(Hepcidin)、膜转铁蛋白(FPN)、二价金属转运蛋白1(DMT1)、十二指肠色素b(DCYTB)是调节铁代谢的重要通道蛋白;本研究通过观察大鼠去势后铁代谢相关通道蛋白基因表达的变化,探讨雌激素与铁代谢的关系。方法雌性大鼠12只,随机分为2组:一组大鼠切除双侧卵巢(去势组),一组切除卵巢周脂肪(对照组),12 w后取标本检测观察;观察指标:①酶联免疫吸附法(Elisa)检测雌二醇浓度,②全自动生化分析仪检测大鼠血清铁,③电感耦合等离子体发射光谱仪原子吸收法检测鼠胫骨铁含量、肝组织铁含量,④半定量RT-PCR法检测肝脏Hepcidin、FPN的表达,以及十二指肠FPN、DMT1、DCYTB的mRNA的表达。结果去势组3项指标(雌二醇19.45±2.28 ng/L、血清铁36.36±9.34μmol/L、胫骨铁含量41.00±5.59μg/g)比对照组相应指标(雌二醇34.98±4.48 ng/L、血清铁54.73±11.08μmol/L、胫骨铁含量66.92±21.94μg/g)显著降低(P<0.05);去势组肝脏铁含量(750.20±94.08μg/g)比对照组(369.60±61.87μg/g)显著升高(P<0.05);RT-PCR结果显示:两组均有肝脏Hepcidin和FPN mRNA表达,表达水平两组比较存在显著差异;两组均有十二指肠FPN、DMT1和DCYTB mRNA表达,其中FPN、DCYTB mRNA表达水平两组比较有显著性差异,DMT1两组比较无明显变化。结论大鼠去势后可以使调控铁代谢的基因通道发生改变,影响铁的摄入和分布。
Objectives In vivo, Hepcidin, FPN, DMT1 and DCYTB are important channel proteins that regulate iron metabolism. In this study, To observe the changes of gene expression related to iron metabolism in rats after castration, and to explore the relationship between estrogen and iron metabolism. Methods Twelve female rats were randomly divided into two groups: one group was ovariectomized, the other was ovariectomized (control group). Twelve weeks later, specimens were taken for observation. Serum estradiol level was detected by Elisa, serum iron was detected by automatic biochemical analyzer, iron content in tibia and iron content in liver tissue were determined by inductively coupled plasma atomic emission spectrometry. The expression of hepatic Hepcidin and FPN, and the expression of FPN, DMT1 and DCYTB in duodenum were detected by RT-PCR. Results Compared with the control group, the indexes of estradiol (estradiol 19.45 ± 2.28 ng / L, serum iron 36.36 ± 9.34 μmol / L, tibia iron content 41.00 ± 5.59 μg / g) (P <0.05). The content of iron in the castration group (750.20 ± 94.08 μg / g) was significantly lower than that in the control group (369.60 ± 61.87μg / g) (P <0.05). RT-PCR results showed that Hepcidin and FPN mRNA were expressed in both groups, the expression levels of which were significantly different between the two groups. Both groups had duodenal FPN, DMT1 and DCYTB mRNA expression, in which FPN, DCYTB mRNA expression levels were significantly different between the two groups, DMT1 no significant change between the two groups. Conclusion Ovariectomy can change the gene pathway regulating iron metabolism and affect the intake and distribution of iron.