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从1981年11月起,作者等在神经科搜集肌肉疾病之肌肉切片样本合计684例,切片样本主要做组织病理,组织化学诊断,或必要时做电子显微镜检查。切片方法通常为经皮切开,或针刺切片。切片之肌肉组织,分为三部分,一者在以液态氮冷至-160℃之isopentane内急速冷冻,以便做组织冷冻切片,病理染色及组织化学染色;另一部分组织培养或生化分析;第三部分则电镜下做超微细构造之观察。电镜检查用之肌肉组织切成1立方毫米小块固定于3％glutaraldehyde内,而后再以锇酸二度固定染色,脱水后渐次包埋於epon内,切1nm之厚片,选择将镜检之部位,切100nm之薄片,并以uranyl acetate及lead citrate双重染色,而后用日立500型电子显微镜观察。
Since November 1981, the authors collected a total of 684 musculoskeletal samples from neurological departments for muscular diseases. The biopsy specimens were mainly histopathologically and histochemically diagnosed, or electron microscopy was performed if necessary. Slice method is usually percutaneous incision, or acupuncture section. Slice the muscle tissue and divide it into three sections, one of which is rapidly frozen in isopentane cooled to -160 ° C with liquid nitrogen for tissue cryosectioning, pathological staining and histochemical staining; the other is tissue culture or biochemical analysis; the third Part of electron microscopy to do ultrafine structure of the observation. Electron microscopy with a piece of muscle tissue cut into 1 cubic millimeter fixed in 3% glutaraldehyde, and then again with osmium acid two fixed staining, dehydration gradually embedded in epon, cut 1nm thick slices, the choice of microscopic examination Site, cut a 100 nm slice, and double-stained with uranyl acetate and lead citrate, followed by observation with a Hitachi 500 electron microscope.