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目的:建立同时测定纤花线纹香茶菜中新西兰牡荆苷2、异夏佛塔苷、夏佛塔苷含量的方法,并分析其含量动态变化。方法:采用Phenomenex luna C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.5%甲酸水溶液,梯度洗脱,流速为0.8ml·min-1,检测波长为330 nm,柱温为室温(25℃),进样量:10μl。结果:新西兰牡荆苷2、异夏佛塔苷、夏佛塔苷线性范围分别为0.130~3.110μg(r=0.999 8)、0.180~4.540μg(r=0.999 9)、0.08~1.97μg(r=0.999 9);平均回收率分别为96.8%、99.2%、97.1%,RSD分别为1.9%、1.6%、1.6%(n=6)。上述3种水溶性黄酮成分从幼苗期开始显著累积,异夏佛塔苷和夏佛塔苷在3月和8月达到最高值;新西兰牡荆苷2的含量在1~4月趋于稳定,5月后开始逐渐累积,8月达最高值,之后开始呈逐渐下降的趋势。结论:以水溶性黄酮成分含量为指标,纤花线纹香茶菜的最佳采收期为3月和8月,且以8月份采收的药材质量最佳。
Objective: To establish a method for the simultaneous determination of vitexin 2, isofravir glycoside, and stilbotide in Sinocapra latifolia L. and analyze its dynamic changes. METHODS: Phenomenex luna C18 (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol-0.5% formic acid and eluted with a gradient of 0.8 ml · min-1. The detection wavelength was 330 nm. Room temperature (25 ℃), injection volume: 10μl. Results: The linear ranges of vitexin 2, isoflavoside and clovetazine were 0.130 ~ 3.110μg (r = 0.999 8), 0.180 ~ 4.540μg (r = 0.999 9) and 0.08 ~ 1.97μg = 0.999 9). The average recoveries were 96.8%, 99.2% and 97.1%, respectively, with RSDs of 1.9%, 1.6% and 1.6% (n = 6), respectively. The above three kinds of water-soluble flavonoids significantly accumulated from seedling stage, the isoflavoside and the summer of fulvestrant reached the highest value in March and August; New Zealand vitexin 2 content in January to April tends to be stable, After May gradually began to accumulate in August reached its highest level, then began to show a gradual downward trend. Conclusion: According to the content of water-soluble flavonoids, the best harvesting period is March and August, and the quality of the medicinal materials harvested in August is the best.